Yeast Bank Starter Procedure

  • February 07, 2013 12:49 PM
    Message # 1201971
    Deleted user
    Just checking to make sure i am doing the yeast bank starter properly.  

    1.  Take yeast slant vial (A) and wort vial (B) out of fridge and bring to room temperature
    2.  Dump oil out of A.
    3.  Pour B into A and Shake.
    4.  Wait 24 hours and step up 5-10X

    Is this correct or sufficient?
    Is the starter volume A+B 50mL?  
    I am currently doing a 0.5L step and there is some agar that has not dissolve all of the way.  Is this normal?

    ***THANKS TOM FOR HAVING THE YEAST BANK!  WHAT AN AWESOME CLUB PERK!***


  • February 12, 2013 6:39 AM
    Reply # 1206747 on 1201971

    You have it almost correct.  The volume of the first starter is only 25ml, as the other 25ml is an Agar mixture, and doesn't count towards the overall total.  Your first step up should then be to 250ml.

    Don't worry about trying to break up the agar mixture, as it will just drop out later.

    Cheers,

    Tom

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